Engineering a Point-of-care Viral Concentration Device for Rapid Molecular Diagnostics of Influenza in Human Respiratory Specimens
نویسندگان
چکیده
We have developed a portable, simple to fabricate, and highly effective polymeric microfluidic sample concentration device to increase the speed and sensitivity of molecular diagnostics of influenza from patient respiratory samples. This method is more friendly than off-chip methods to in-line on chip processing since it obviates the need for a centrifuge. The disposable chip fabricated with xurography utilizes evaporation and interfacial dragging effects. Up to 18 times concentration for viral RNA from influenza A virions was achieved by evaporating 1 mL to 50 μL in 0.5 hour or less with an average recovery of 60.3% (+/-20.5%) from patient specimens.
منابع مشابه
Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses
Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. ...
متن کاملThe Potential Effect of Glycyrrhiza Glabra on Early Step of Influenza Virus Replication
Background and Aims: The emergence of drug-resistant influenza viruses has become a serious threat for human and animal populations. Glycyrrhiza glabra (Gg) is a traditional medicine clinically used for the treatment of viral respiratory infection symptoms in most countries. We evaluated the effects of the herb on influenza virus replication in human lung cultured cells (A549) following the det...
متن کاملDetection of avian influenza virus of H9 subtype in the feces of experimentally infected chickens by RT–PCR
Avian Influenza (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, ...
متن کاملSensitivity of the Quidel Sofia Fluorescent Immunoassay Compared With 2 Nucleic Acid Assays and Viral Culture to Detect Pandemic Influenza A(H1N1)pdm09.
To confirm a diagnosis of influenza at the point of care, healthcare professionals may rely on rapid influenza diagnostic tests (RIDTs). RIDTs have low to moderate sensitivity compared with viral culture or real-time reverse-transcription polymerase chain reaction (rRT-PCR). With the resurgence of the influenza A (Flu A; subtype H1N1) pandemic 2009 (pdm09) strain in the years 2013 and 2014, we ...
متن کاملMolecular and Phylogenetic Analysis and Protein Structural modeling of NS Gene of Human Influenza A Virus Subtype H1N1 Circulating in Iran 2015 & 2017
Abstract Background: The NS (non-structural) genomic segment of influenza A virus expresses two proteins (NS1 and NS2) which are responsible for the virulence and pathogenicity of virus. In this study we investigate the characterization and variability of the NS gene recovered from H1N1 influenza viruses isolated from Iranian patients during the 2017 seasonal outbreak and from high...
متن کامل